1. Captain Nemo: Organ
2. Sam: Piano ("Casablanca")
3. Elwood Blues: Harmonica
4. Kwai Chang Caine: Flute
5. Mr. Scott: Bagpipes (remember Spock's funeral at the end of Star Trek II?)
6. Holly Golightly: Guitar
7. Snorky: Organ and guitar
8. Dr. Charles Emerson Winchester III: French Horn
9. Squidward: Clarinet
10. Kermit the Frog: Banjo
11. Ziggy Stardust: Guitar
12. Lisa Simpson: Saxophone
13. Grumpy: Pipe organ
14. Richie Cunningham: Saxophone
15. Edith Bunker: Piano
16. Geppetto: Accordion
17. Dirk McQuickly: Bass (The Rutles)

Bonus: Spider Murphy and Little Joey: "Spider Murphy played the tenor saxophone, Little Joey blowin' on the slide trombone" (Elvis, "Jailhouse Rock")